PSVI-7 Postmortem in Vitro Culture of Cells from Visceral Organs Stored at 4°C in Small Ruminants

Abstract Exploration of cell viability and their regenerative capacity in postmortem tissues has a huge potential for tissue/organ transplantation therapies both human animals. Proliferative stem cells have been recovered from refrigerated skin tissue bovine, goat sheep, ligaments horses. However, it is not clearly known whether live proliferative can be visceral organs that stay comparatively warmer than other after death, the recovery time extended refrigeration individual organs. To achieve this goal, six different organ i.e. lung, heart, oviduct, liver, kidney goats were procured slaughter house stored laboratory at 4°C. Ten small sized (2-3mm2) explants each five then cultured two 35mm dishes point DMEM media supplemented with 10% fetal bovine serum. The on day 0, 3, 6, 9 12. observed under inverted microscope any explant exhibiting outgrowth 10-12 culture was considered positive. Our results exhibited around 3 (lung, skin) 6 tested. Kidney, liver heart did show outgrowth. Interestingly, oviduct skin, even days storage refrigerator, whereas lung growth only up to storage. Experiments test survival limits more animals age groups characterization are underway.